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The substrate Option was then stored at −20°C until finally the ammonium and nitrate contents had been analyzed While using the San++ Constant-Circulation Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate willpower was determined by a response with hydrazinium sulfate, sulfanilamide and N-(one-naphthyl) ethylenediamine dihydrochlorid

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